The Lineweaver-Burk plot is a graphical representation of the Michaelis-Menten equation, which describes the relationship between the reaction velocity and the substrate concentration in an enzyme-catalyzed reaction. The initial velocity is the velocity of the reaction at the beginning, when the substrate concentration is zero. To find the initial velocity on a Lineweaver-Burk plot, you can extrapolate the linear portion of the curve to the y-axis. The y-intercept of this line will give you the initial velocity.
The Lineweaver-Burk plot is a useful tool for determining the kinetic parameters of an enzyme-catalyzed reaction. The Michaelis constant (Km) is the substrate concentration at which the reaction velocity is half of the maximum velocity. The Vmax is the maximum velocity of the reaction. The Lineweaver-Burk plot can also be used to determine the type of inhibition that is occurring in an enzyme-catalyzed reaction.
